PickMol technology applied for multiplexing chip used for direct extractionless detections of pesticide molecules in milk was validated according to environmental test methods (CEN guide 2013)

PickMol technology applied for multiplexing chip used for direct extractionless detections of pesticide molecules in milk was validated according to environmental test methods (CEN guide 2013)

SAFTRA photonics has completed the procedure for the standardisation process of the PickMolTM technology to verify it as an approved reference method for high-sensitive screening of Persistent Organic Pollutants (POPs) (pollutant molecules) in MILK SAMPLES in a mode of MULTIPLEXING ANALYSIS and DIRECT EXTRACTION-LESS DETECTION.

The validation tasks were performed according to the “Validation of environmental test methods CEN guide 2013”. The procedure has presented the reliability of particular measurement steps based on the comparative trials performed by validated HPLC-MS-MS technology.

Based on the previous validations [1],[2], the optimized nano-optical multiplexing chips (PickMolTM Sensing Chip), detection system PickMolTM RAMASCOPE, measurement and evaluation software PickMolTM sw/app, and database of pure forms of detected molecules[3] (PickMolTM database), three different molecules (Paraquat – PQ, Diquat – DQ and Thiram –TR) and six different – randomly selected – commercially available milks (with different milk fat content) were tested to be simultaneously detected in the solutions of the PQDQTR (milk) mixture at concentration level 10-6 M. These solutions were analysed at the same time by HPLC-MS-MS and PickMolTM technology.

By PickMolTM technology 3 measurements (with 3 different chips) for every milk, pesticide’ stock and final mixture’ solutions were carried out. The measurements were repeated for each “Parallel”. In total, the presence of PQ, DQ and TR in milk (10-6 M PQDQTR (milk)) on 54 different chips have been tested. The blank or background sample measurements were provided with the use of pure milk samples (without the pesticide molecules).

The technical report also represents a validation procedure performed by HPLC-MS-MS. The validation process by HPLC-MS-MS required laborious sample preparation and 2 different HPLC-MS-MS condition setups for 3 pesticide molecules (one for Paraquat and Diquat, one for Thiram) to confirm the presence of the pesticides in the tested milk samples.

Conclusions

The technical report demonstrates the standardisation process of PickMolTM technology applied for multiplexing chip based on the verification by HPLC-MS-MS as a standard validated method for POPs/pesticide molecules analyses in milk.

PickMolTM technology is shown as a powerful screening tool for ultrafast analyses for milk contamination. Complete measurement protocol from sampling to data processing is achieved within 10 min and real sample analysis time is ~ 2 minutes.

 

Validation report available upon request: roman.oros@saftra-photonics.org

[1] https://www.saftra-photonics.org/pickmol-technology-applied-for-multiplexing-chip-was-validated-according-to-environmental-test-methods-cen-guide-2013/

[2] https://www.saftra-photonics.org/pickmol-validated-according-to-environmental-test-methods-cen-guide-2013/

[3] http://db.pickmol.tech/#/database

PickMol technology applied for direct detection of pesticides in milk samples

PickMol technology applied for direct detection of pesticides in milk samples

SAFTRA photonics has already performed the test measurements to provide the procedure for the standardization process of the PickMolTM technology to verify it as an approved reference method for high-sensitive screening of pollutant molecules in milk samples. The measurements were carried out in accordance with validation tasks performed previously according to the “Validation of environmental test methods CEN guide 2013” The PickMolTM technology, based on plasmonic-enhanced Raman scattering (PERS) and represented by a construction of particular topology/structure of nano-optical chips, was used to detect sensitively both the selected pesticide molecule (5×10-7 M Paraquat) as well as the mixture of pesticides (Paraquat – PQ, Diquat – DQ and Thiram – TR; 5×10-7 M PQDQTR) in a mode of multiplexing analysis in milk. We have randomly tested six commercially available milks with different milk fat content. The “Blank” sample measurements were provided with the use of pure milk (in the absence of pesticide molecule(s)). PickMolTM technology was shown to be a powerful screening tool for direct and ultrafast analyses of the selected pesticides found in milk samples. The presence of target molecule(s) (PQ, PQDQTR) in milk samples was confirmed by characteristic Raman bands evidenced by pattern recognition analysis. It was demonstrated that PickMolTM Sensing Chip is capable to detect all three molecules directly in different kinds of milk (other molecules detectable directly in milk are listed in our databases).  Complete measurement protocol from sampling to data processing is achieved within 10 min and real samples analysis time is 2 minutes. In addition, higher sensitivity (at least of 1 order) can be achieved by simple pre-treatment process of the pollutant-milk sample.

PickMol technology applied for “multiplexing chip” was validated according to environmental test methods (CEN guide 2013)

PickMol technology applied for “multiplexing chip” was validated according to environmental test methods (CEN guide 2013)

SAFTRA photonics has also completed the procedure for the standardisation process of the PickMolTM technology to verify it as an approved reference method for high-sensitive screening of Persistent Organic Pollutants (POPs) (pollutant molecules) in water samples in a mode of MULTIPLEXING ANALYSIS. The validation tasks were performed according to the “Validation of environmental test methods CEN guide 2013”. The procedure has presented the reliability of particular measurement steps based on the comparative trials performed by validated LCMS technology.

The PickMolTM technology is based on plasmonic-enhanced Raman scattering (PERS) and is represented by a construction of particular topology/structure of nano-optical chip. The “multiplexing chip” is capable to detect multiple organic molecules with different concentration level in water sample. Three different molecules (Paraquat – PQ, Diquat – DQ and Thiram – TR) were tested to be simultaneously detected in the solutions of the PQDQTR (aq) mixture at different concentrations (from 10-5 M to 10-7; PQ: DQ: TR = 1: 1: 1). These solutions were analysed by the same time by HPLC-MS-MS and PickMolTM technology. We have carried out 3 measurements (with 3 different PickMol chips) for every stock and working solution. The measurements were repeated for each “Parallel”. In total, we have tested the presence of PQ, DQ and TR (of different concentration) on 30 different chips. The “Blank” sample measurements were provided with the use of pure (Milli-Q) water.  

Conclusions

Technical report demonstrates the standardisation process of PickMolTM technology applied for multiplexing chip based on the verification by LCMS-QTRAP hybrid technology as standard validated method for POPs/pesticides analyses in water. PickMolTM technology is shown as powerful screening tool for ultrafast analyses in the environment. Complete measurement protocol from sampling to data processing is achieved within 10 min and real sample analysis time is 2 minutes.

The confirmation of target analyte (pollutant) is performed by the presence of characteristic Raman bands of the detected molecules. Two different HPLC-MS-MS conditions /mobile phase composition and gradient elution profile/had to be used first for identification PQ, DQ and second one for Thiram analyses. The single analysis performed by PickMolTM multiplexing nano-optical chip clearly demonstrates the benefits in term of sample preparation costs, equilibration time and price of analysis.

The technical report represents a validation procedure for “multiplexing chip” which is capable to detect multiple organic molecules with different concentration level in water sample. At higher concentrations (10-5 M and 10-6 M) of the analysed PQDQTR (aq) solution (PQ: DQ: TR = 1: 1: 1), all three molecules (PQ, DQ, TR) were positively detected (the intensities of their characteristic Raman bands were above the set 3Sigma limit) by means of PickMolTM technology. PQ and DQ were observed/detected in the Raman spectra recorded for the PQDQTR (aq) mixture at 10-7 M concentration as well. In case of Thiram, the screening threshold of employed nano-optical chip appeared at 10-6 M concentration of analyte.

 

Validation report available upon request: roman.oros@saftra-photonics.org

PickMol is the new product brand

PickMol is the new product brand

Global expansion of SAFTRA photonics has challenged the product name, that has gained multiple successes for the company. Unfortunately, NanoScreen was not a globally protectable trademark. The new name PickMol is a beginning of a new era, when the company has grown from a spin-off to a company economically active on market introducing its solutions to customers. The creative process has led to a new name PickMol. It reflects the qualities of product able to pick organic molecules even in complex water solutions and indicate their presence. Another interpretation is the target sensitivity of the super-fast and super-sensitive solution being developed to detect up to pico-molar concentration. In writing, the name is spelled with major P and major M. The new logo refers to the symbol of nano-sensing chip, the core of product innovation.

Startup Awards 2017 has boosted the company potential

Startup Awards 2017 has boosted the company potential

Exactly a year ago, SAFTRA photonics has been awarded the FutureNow Startup Awards 2017, the most prestigious Slovak early entrepreneur prize. It has been a great achievement and boost for the company potential. In the period prior to the final event, we experienced the boot camp – a weekend-long gathering of start-ups, mentors and investors. It brought new views on the business potential of our solution and opened unexpected directions of the business plan. Also, the experience of 3-minute pitching was first of its kind for us. The real scientists are not used to explain 25 years of technology development in just one slide ? The final event and months that followed were very special for us. Intense preparation and great solution were showcased on stage and the jury consisting of senior investors has fully accepted it! The interest raised by the awards was a test of our media preparedness and investment strategies. Now, we are about to sign a significant investment and still occupy the media attention. Moreover, thanks to sponsors and donations related to the main prize, we were able to address a new market in the USA – special thanks to Slovak-American Foundation for their $35 000 contribution and to SOVVA for their 10 000 EUR contribution used to support our product development efforts.

End-to-end detection system introduced

End-to-end detection system introduced

First of its kind analytical detection system for low concentrations of organic substances in water was introduced by SAFTRA photonics. As the company CEO Pavol Miskovsky said, it is a milestone for the worldwide analytical industry.  

By scaling a complex laboratory for water pollution testing into a suitcase size the detection system brings the analysis to the source and allows great flexibility, speed and sensitivity for customers and analytical service providers. The sensitivity thresholds can be set up to part-per-billion – allowing addressing most critical applications in drinking water supplies, food and beverage industry or environmental protection. The system builds on plasmon nano-photonics technology (patent pending) and innovative Raman detection system that allows selective detection of trace quantities of organic molecules in a different environment. Water is the first target medium.

The system will be presented for the first time to the public on the China International Industry Fair in Shanghai on 19-23 September 2018. Please address the test case requests on: maros.chromik(at)saftra-photonics.org